Raman is a label-free analytical method, which offers tremendous advantages for biomolecular detection. Surface-enhanced Raman scattering (SERS) technique can overcome the low cross-sectional problems inherent in Raman spectroscopy. SERS has been observed for a very large number of molecules adsorbed on the surface of Au or Ag in a variety of morphologies and physical environments. With these environments, its detection limit can reach up to 6-10 orders of magnitude over conventional Raman spectroscopy. We know nanoparticle sizes (15-200 nm) and interparticle spaces (0-10 nm) are crucial for large enhancement factors. Despite of these advantages and lots of studies so far, method of embedding the crucial sized nanostructures into microfluidics has not been established. Here we introduced nanofabrication using random sequential adsorption of Au nanoparticles and room-temperature seeding of them in microfluidics.
Project end date: 02/07/06