Development of reliable glucose sensors for noninvasive monitoring without interruption or limiting users’ mobility is highly desirable, especially for diabetes diagnostic which requires routine/long term monitoring. However, their applications are largely limited by the relatively poor stability. Herein, a porous membrane is synthesized for effective enzymes immobilization and it is robustly anchored to the modified nanotextured electrode solid contacts, so as to realize glucose sensors with significantly enhanced sensing stability and mechanical robustness. To the best of our knowledge, it is the first report on utilizing such nanoporous membranes for electrochemical sensor applications, which eliminates enzymes escaping and provides sufficient surface area for molecular/ion diffusion and interactions, thus to ensure the sustainable catalytic activities of the sensors and generate reliable measureable signals during noninvasive monitoring. The as-assembled nanostructured glucose sensors demonstrates reliable long-term stable monitoring with minimal response drift for up to 20 hours, which delivered a remarkable enhancement. Moreover, they can be integrated into a microfluidic sensing patch for noninvasive sweat glucose monitoring. The as-synthesized nanostructured glucose sensors with remarkable stability can inspire developments of various enzymatic biosensors for reliable noninvasive composition analysis and their ultimate applications in predictive clinical diagnostics, personalized healthcare monitoring and chronic diseases management.
Project end date: 08/14/20