Platelets are stimulated by mechanochemical cues to trigger hemostatic and regenerative responses. Platelet activation, especially in the case of clotting, is generally thought of to be caused by shear- based forces. However, clinical observation suggests that pressure plays an influential role in the mechanical microenvironment. To probe the ways and extent to which pressure influences platelet activation and secretion of factors, we will develop a series of microfluidic devices to apply various magnitudes, gradients, and frequencies of pressure stimuli onto human platelets. Results may explain differences in platelet behavior in patients of varying cardiovascular health and as a result of physical strain.
Project end date: 01/29/20