We present the design, fabrication and results of microfabricated interfaces for the patterned delivery of foreign molecules via electroporation into developing embryos. We show how these systems can be used to â€˜drawâ€™ two-dimensional patterns of tracer molecules, DNA and mRNA into the yolk and cells of zebrafish embryos (Danio rerio) at different stages of development. We demonstrate the successful delivery of two-dimensional patterns of trypan blue (normal dye), texas red (fluorescent dye), pCS2eGFP DNA and GFP-mRNA in both chorionated and dechorionated embryos. Both DNA and mRNA were expressed in the desired patterns subsequent to delivery.
Project end date: 08/11/09