BPN450: A Microsystem for Sensing and Patterning Oxidative Microgradients During Cell Culture

We present a microsystem capable of electrochemically patterning dissolved oxygen gradients during cell cultures. Multiple electrodes in an array each generate distinct amounts of dissolved oxygen via electrolysis; these different sources superimpose to generate one- and two-dimensional microgradient profiles not possible with other methods. We believe this is the first technology that enables researchers to pattern localized oxygen doses and program arbitrary oxygen gradients with microscale resolution during cell culture.

Project end date: 02/03/10