BPN937: Nanoplasmonic PCR-based Rapid Precision Molecular Diagnostic Chip


Emerging molecular diagnosis requires ultrafast polymerase chain reaction (PCR) on chip for rapid precise detection of infectious diseases, neurodegenerative diseases, cardiovascular diseases, or cancers. Here we report nanoplasmonic PCR-based rapid precision molecular diagnostic chip. The nanoplasmonic pillar arrays (NAPA) comprise gold nanoislands on the top and sidewall of large-scale glass nanopillar arrays. The nanoplasmonic pillars enhance light absorption of a white light- emitting diode (LED) over the whole visible range due to strong electromagnetic hotspots between the nanoislands. As a result, they effectively induce photothermal heating for ultrafast PCR thermal cycling. The temperature profile of NAPA exhibit 30 cycles between 98 ℃ and 60 ℃ for total 3 min 30 sec during the cyclic excitation of white LED light. The experimental results also demonstrate the rapid DNA amplification of both 0.1 ng μl-1 of λ-DNA in 20 thermal cycles and 0.1 ng μl-1 of complementary DNA of Middle East respiratory syndrome coronavirus in 30 thermal cycles using a conventional PCR volume of 15 μl. Our nanoplasmonic PCR chip provides a new opportunity for rapid precision molecular diagnostics.

Project end date: 06/01/20

Publication date: 
January 27, 2020
Publication type: 
BSAC Project Materials (Final/Archive)
PREPUBLICATION DATA - ©University of California 2020

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