Dengue fever is one of global health concerns as two fifth of world population are considered to expose to the infection risk and 20K patients results in death per year. Even after successful recovery from the febrile disease, it often causes secondary neurological complications including encephalopathy, residual brain damage and seizures. However, unclear etiological details of neurological disorders still inhibit to uncover suitable treatments of the complications. Herein, we develop an in-vitro brain model for the comprehensive systematic analysis of neurological complications due to of the Dengue infections. Our new minibrains-on- a-chip concept will allow to mature brain tissue mimicking in-vivo tissue complex in an interstitial fluidic dynamics and to monitor electrophysiological phenotypes in an in-situ manner. By administrating various etiological factors found in dengue virus (DENV) infection along with the dynamic flow, we will be able to address phenotypic connections of individual etiological factors. Our integrated in- vitro brain model analysis platform for Dengue infections will potentially provide a diagnostic and therapeutic frame for various levels of neurological disease associated with DENV infection.
Project end date: 08/08/17