BPN495: QES: Continuous Flow Cell Lysometer

Single cell analysis is an increasingly important area of consideration. Rather than obtaining a bulk average assay result from a large number of cells, it is possible to do a statistical study on each individual cell in a population. Flow cytometry allows this methodology, but is incapable of testing for compounds inside the cells themselves. It instead relies on using surface markers. Some limited markers (calcium probes) capable of penetrating the cell wall are also available, but are limited to a few very specific tests. Our device will enable the assay of cytosolic (internal) components in individual cells in a rapid, continuous manner.

Project end date: 01/30/13