Luke P. Lee (Advisor)

BPN824: Investigation of Dengue Infection’s Neurological Complications via a Comprehensive In Vitro Brain Model

SoonGweon Hong
Minsun Song
2017

Dengue fever is one of global health concerns as two fifth of world population are considered to expose to the infection risk and 20K patients results in death per year. Even after successful recovery from the febrile disease, it often causes secondary neurological complications including encephalopathy, residual brain damage and seizures. However, unclear etiological details of neurological disorders still inhibit to uncover suitable treatments of the complications. Herein, we develop an in-vitro brain model for the comprehensive systematic analysis of neurological complications due...

BPN836: Nanocrescent Antenna for Nanofocusing of Excitation Radiation and Concentrate Upconversion Emission

Doyeon Bang
2017

Frequency upconversion activated with Lanthanide has attracted attention in various real-world applications, because it is far simpler and more effective than traditional nonlinear susceptibility-based frequency upconversion, such as second harmonic generation. However, the quantum yield of frequency upconversion of Lanthanide-based upconversion nanoparticles remains inefficient for practical applications, and spatial control of upconverted emission is not yet developed. To overcome this limitation, we developed asymmetric hetero-plasmonic nanoparticles (AHPNs) consisting of...

BPN829: Integrated Multiplexed Optical Microfluidic System (iMOMs) for Dengue Diagnosis

Jong-Hwan Lee
Jun Ho Son
2018

Dengue is an endemic viral disease that affects tropical and subtropical areas. It is estimated that more than 50 million infections occur worldwide per year. Due to its lack of pathognomonic clinical features, dengue is often mistakenly diagnosed as other febrile diseases, which thus leads to ineffective and costly overtreatment. Previously developed diagnostic tests for dengue can only detect a single biomarker (or two to three kinds of targets) at a time and they also lack comprehensive and syntagmatic analysis between various dengue-specific biomarkers. For an effective and...

BPN906: Integrated Zebrafish Analysis Platform (iZAP) for the Systematic Study of Neurological Disorders and Drug Discovery

Wonseok Kim
Youngseop Lee
2019

A vertebrate model, zebrafish is popularly used to investigate biological systems, especially for neurological disorders and drug discovery. This animal has experimental advantages such as fecundity, breeding convenience, genetic homology to human, and optical transparency. However, laborious and invasive procedures have been required for electrophysiological studies for brain investigation. In this project, we developed an Integrated Zebrafish Analysis Platform (iZAP) for the systematic analysis of neurological disorders and drug discovery. The iZAP is an integrated electrical...

BPN809: Online Optical Regulation and Photocurrent Sensor for Monitoring Microalgal Bioreactor System

Wonseok Kim
Youngseop Lee
2019

Optogenetic technology, which utilizes light to control genetically encoded light sensitive ion channels and pumps, has made a significant impact on neuroscience and neurotechnology since it allows to modulate selective neurons. Even though it is an ideal technology for numerous biological researches, irreversible and permanent gene modifications of biological tissues by lentriviral vectors in optogenetics hinder many real...

BPN923: Rapid Isolation and Identification of Drug-Resistant Pathogens with Smartphone-Based Optical System (SOS)

Wonseok Kim
Youngseop Lee
2020

The rapid isolation and identification of antimicrobial-resistant pathogens are critically demanded in global healthcare and precision medicine. However, time-consuming, culture-based methods are currently used for the detection of pathogens since there is no rapid precision molecular diagnostic platform. In this work, we develop a rapid isolation and identification of drug-resistant pathogens with smartphone-based optical system (SOS). For the pathogens detection from human blood sample, we precipitate RBCs with pre-mixed dextran for 10...

BPN937: Nanoplasmonic PCR-based Rapid Precision Molecular Diagnostic Chip

Youngseop Lee
Wonseok Kim
2020

Emerging molecular diagnosis requires ultrafast polymerase chain reaction (PCR) on chip for rapid precise detection of infectious diseases, neurodegenerative diseases, cardiovascular diseases, or cancers. Here we report nanoplasmonic PCR-based rapid precision molecular diagnostic chip. The nanoplasmonic pillar arrays (NAPA) comprise gold nanoislands on the top and sidewall of large-scale glass nanopillar arrays. The nanoplasmonic pillars enhance light absorption of a white light- emitting diode (LED) over the whole visible range due to strong...

Microfluidic Technologies for Single Cell Analysis

Dino Di Carlo
Luke P. Lee
Jay T. Groves
Tejal A. Desai
2006
Analysis of single cells is important for a complete understanding of the heterogeneity that is present in cell behavior and function. Current single cell analysis methods provide high throughput information about labeled compounds within cells, but in most cases cannot follow the dynamic processes occurring in signaling pathways. New microfluidic methods have also been developed to assay fast timescale responses or create uniform environments to study cell behavior in a more quantitative manner....

Massively Parallel Optical Manipulation of Single Cells, Micro- and Nano-particles on Optoelectronic Devices

Pei-Yu Chiou
Ming C. Wu
Connie J. Chang-Hasnain
Luke P. Lee
2005
The ability to manipulate biological cells and micrometer-scale particles plays an important role in many biological and colloidal science applications. However, conventional manipulation techniques, such as optical tweezers, electrokinetic forces (electrophoresis, dielectrophoresis (DEP), and traveling-wave dielectrophoresis), magnetic tweezers, acoustic traps, and hydrodynamic flows, cannot achieve high resolution and high...

Integrated Biophysical Microfluidic Platforms for Pathogen Detection and Single Cell Analysis

Poorya Sabounchi
Luke P. Lee
Albert P. Pisano
Liwei Lin
Daniel A. Fletcher
2007
Pathogen detection is of the utmost importance primarily for health and safety reasons. The most common problem encountered with current pathogen monitoring techniques is the inverse relationship between specificity versus sensitivity and speed. Current tools used for pathogen identification are accurate but require a vast amount of sample preparation time, typically from an experienced operator in a laboratory setting and therefore not suitable for...