Prof. Rong Fan
Associate Professor, Department of Biomedical Engineering, Yale University
October 13, 2015 | 12:00 to 01:00 | 540 Cory Hall
Host: Luke Lee
Despite recent advances in single-cell genomic, transcriptional and mass cytometric profiling, it remains a challenge to collect highly multiplexed measurements of proteins produced from single cells for comprehensive analysis of immune functional state. We combine spatial and spectral encoding with ultra-high density antibody microarrays patterned in sub-nanoliter PDMS microchambers for co-detection of 42 immune effector proteins secreted from single cells, representing the highest multiplexing recorded to date for a single-cell secretion assay. Using this platform to profile human macrophages stimulated with pathogenic ligands reveals previously unobserved deep functional heterogeneity and varying levels of pathogenic activation. Surprisingly the subpopulation architecture is highly conserved throughout the cell activation process and collectively controls the homeostasis of macrophage-mediated innate immune response. A subset macrophage cells that display multiple effector functions, named polyfunctional population, are the "super warriors" dominating the immune defense against pathogens. This technology was also applied to examining the production of these immune modulatory factors in hematopoietic cells and their role in hematologic malignancy. Again, we identified the existence of polyfunctional subpopulation and this population is markedly increased in myeloproliferative disorders. Interestingly, both cancerous and "normal" hematopoietic cells in the disease compartment show increased polyfunctionality and skewed cytokine programs. Unexpectedly, these "normal" hematopoietic cells with abnormal cytokine functions contribute substantially to pathogenesis and therapeutic response. All these results underscore the complexity and multifunctionality of phenotypically similar cell repertoire in both protective immune defense and pathological conditions that, however, have not been precisely delineated due to the lack of single cell, high-plex cytokine function analysis tools. Our technology permits a full-spectrum dissection of the immune functional states at the single-cell level and represents an enabling tool for next-generation clinical immune monitoring.
seas.yale.edu/faculty-research/faculty-directory/rong-fan
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