Dr. Youngseop Lee
Department of Bioengineering, UC Berkeley
BSAC Postdoctoral Researcher, Luke P. Lee Group
June 11, 2019 | 12:00 to 01:00 | 490 Cory Hall
Host: Michael Cable
This work presents the novel nanoplasmonic PCR on chip for rapid precision molecular diagnostics. The nanoplasmonic pillar arrays (NPA) comprise gold nanoislands on the top and sidewall of glass nanopillar arrays. The nanoplasmonic pillars enhance light absorption of a white light-emitting diode (LED) over the whole visible range due to strong electromagnetic hotspots between nanoislands. As a result, NPAs allow to induce ultrafast photothermal cycling effectively during the PCR. The temperature profile of NPA exhibit 30 cycles between 98 ℃ and 60 ℃ for total 3 min 30 sec with volume of xxx reactor during the cyclic excitation of white LED light. We demonstrate the rapid DNA amplification of 0.1 ng μl-1 of λ-DNA in 20 thermal cycles as well as 0.1 ng μl-1 of complementary DNA of Middle East respiratory syndrome coronavirus in 30 thermal cycles (time?). Our nanoplasmonic PCR can provide a solution for ultrafast precision molecular diagnostics.
biopoems.berkeley.edu | bsac.berkeley.edu/rsscast
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