Publications

Nanoplasmonics-enabled On-Demand and Systematic Gene Regulation

Eunice Lee
Luke P. Lee
Michel M. Maharbiz
Tejal Desai
Han Lim
2010

In this dissertation, nanoplasmonic optical antennae are utilized as “nanoplasmonic gene switches” for on-demand and systematic gene regulation in living systems. The plasmon resonance of nanoplasmonic gene switches is specifically tuned to the near- infrared spectral region where cells and tissues are essentially transparent. Due to their extraordinarily large surface-to-volume ratio, nanoplasmonic gene switches are ideal carriers of interfering oligonucleotides, such as antisense DNA and short interfering RNA oligonucleotides (siRNA). Interfering oligonucleotides enable direct,...

Biologically-inspired Microfluidic Platforms and Aptamer-based Nanobiosensors

Hansang Cho
Luke P. Lee
Dorian Liepmann
Ming C. Wu
John Kurhanewicz
2010

Recent advances in micro/nano- technologies have shown high potentials in the field of quantitative biology, biomedical science, and analytical chemistry. However, micro/nano fluidics still requires multi-layered structures, complex plumbing/tubing, and external equipments for large-scale applications and nanotechnology-based sensors demand high cost. Interestingly, nature has much simpler and more effective solutions. The goal of this dissertation is to develop novel microfluidic platforms and nanobiosensors inspired by biological systems.

In this dissertation, I...

High-Q Aluminum Nitride RF MEMS Lamb Wave Resonators and Narrowband Filters

Ernest Ting-Ta Yen
Albert P. Pisano
Clark T.-C. Nguyen
Liwei Lin
Richard M. White
2012

The increasing demands for higher performance, advanced wireless and mobile communication systems have continuously driven device innovations and system improvements. In order to reduce power consumption and integration complexity, radio frequency (RF) microelectromechanical systems (MEMS) resonators and filters have been considered as direct replacements for off-chip passive components. In this dissertation, a new radio architecture for direct channel selection is explored. The primary elements in this new architecture include a multitude of closely-spaced narrowband filters (...

Technologies for Blood Diagnostics

John Waldeisen
Luke P. Lee
Zev J. Gartner
Lee W. Riley
2012

For over three decades, the lateral flow assay (LFA) has remained the diagnostic gold standard for immunodetection. In the developed world, the diverse capabilities of these assays is relatively unknown. However in resource-limited settings, LFAs are the best diagnostic tool a clinician currently has other than a microscope to diagnose disease. Although most diseases are treatable and preventable, three diseases alone are responsible for killing more than 5 million people each year: HIV, malaria, and tuberculosis (TB). A devastating lack of diagnostic accessibility in the developing...

Design Considerations for CMOS-Integrated Hall-effect Magnetic Bead Detectors for Biosensor Applications

Karl Skucha
Bernhard E. Boser
Ming C. Wu
Luke P. Lee
2012

This dissertation presents a design methodology for on-chip magnetic bead label detectors based on Hall-effect sensors to be used for biosensor applications. Signal errors caused by the label-binding process and other factors that place constraints on the minimum detector area are quantified and adjusted to meet assay accuracy standards. The methodology is demonstrated by designing an 8,192 element Hall sensor array implemented in a commercial 0.18 μm CMOS process with single mask post-processing. The array can quantify a one percent surface coverage of 2.8 μm beads in thirty seconds...

Thin Film Encapsulation Methods for Large Area MEMS Packaging

Armon Mahajerin
Liwei Lin
Dorian Liepmann
Tsu-Jae King Liu
2012

The past thirty years have seen rapid growth in products and technologies based on microelectromechanical systems (MEMS). However, one of the limiting factors in commercializing MEMS devices is packaging, which can be the most costly step in the manufacturing process. A MEMS package must protect the movable parts of the device while allowing it to interact with its surroundings. In addition, the miniaturization of sensors and actuators has made it possible to integrate MEMS fabrication with that of integrated circuit (IC) processing. Due to the varying requirements for different...

A Cyber-infrastructure for the Measurement and Estimation of Large-scale Hydrologic Processes

Branko Kerkez
Steven D. Glaser
Alexandre M. Bayen
Kristofer S.J. Pister
2012

Water shortages, particularly evident in the state of California, emphasize the need for a better hydrologic understanding, and improved water management techniques. The majority of the state’s water originates in the Sierra Nevada as snow, melting throughout the year to meet the needs of various stakeholders. Current measurement techniques are unable to resolve variability of the snowpack at the basin scale, and snowmelt processes are not well captured by existing hydrologic models. A system-level solution is introduced to facilitate scientific understanding and water management...

Cellular Mechanotransduction via Microfabricated Post Arrays

Adrienne Higa
Liwei Lin
Song Li
Dorian Liepmann
2012

Substrate-based biophysical cues, which interact with cells through mechanotransductive pathways, influence many biological processes and cellular behaviors. By leveraging microfabrication techniques, this work aims to build biophysical stimuli into cellular substrates through discrete microtopographic features to study cellular responses. Here, uniform and gradient arrays of circular microposts have been geometrically tuned to change the apparent rigidity of a substrate and the placement of available cellular attachment sites. Three areas of cellular interaction with these micropost...

Nanomaterial Synthesis and Integration for Sensor and Energy Applications

Heather Chiamori
Liwei Lin
Samuel S. Mao
John Clarke
Oscar D. Dubo ́n
2012

Nanostructures have unique properties that will be leveraged for next generation sensor and energy applications: large surface-to-volume ratios, increased sensitivity at lower input power levels, and higher current densities all contribute to increased functionality of nano- structure devices. The synthesis and integration of carbon-, metal-, and metal oxide-based nanostructures for sensor and energy applications are studied with the goal of nano-to-micro devices utilizing well-established micro- and nano-fabrication tools and techniques: (1) self- assembled, integrated carbon...

Microengineered Platforms For Assessing Cell-Cell Communication and Single-Cell Influences on Adult Neural Stem Cell Fate

Sisi (Siyu) Chen
Michel M. Maharbiz
David V. Schaffer
Henk Roelink
Zev Gartner
2012

The signaling environment experienced by a single cell is highly dependent on its interactions with its neighbors, which may secrete locally acting factors or act via membrane bound receptor-ligand systems. Stem cells are particularly sensitive to these signals, which act in concert to regulate self-renewal and discrete transitions into distinct fates. In the case of the adult neural stem cell, decades of in vivo and in vitro work have illustrated specific roles for different types of niche cells and some of the molecular mediators...